Home > Science > Biology > Cell Biology > Methods and Techniques > Cell Culture
Cells culture is an important field of cell biology for the study of cell physiology, life cycle and apoptosis (in cancer research for example). Cell culture techniques are also used for protein expression in the biotechnology domain, in gene therapy studies, in molecular biology, and for tissue engineering.
http://www.cellculturechat.com/
Bulletin board for the discussion of cell line and methodology, maintained by an instrument distributor Spectrum Laboratories, Inc., Rancho Dominguez, California.
http://www.doubling-time.com/
Downloadable software to compute the doubling time of a cell line, and curve fitting to estimate cell concentration as function of time. [English/Chinese]
http://pfaff.salk.edu/research/protocols/
Introduction to the steps and reagents involved in tissue culture, at the Pfaff Lab, Salk Institute, San Diego, California.
http://userpages.umbc.edu/~jwolf/method5.htm
Describes the equipment and work space requirements for cell and tissue growth. Includes details on cryopreservation, maintenance, media prep procedures, safety considerations and link to the masters degree at University of Maryland Baltimore County.
http://www.worthington-biochem.com/tissuedissociation/
Resource on cell isolation theory and techniques, including use-tested systems, FAQ, and annotated references searchable by multiple criteria from Lakewood, NJ.
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