Directory of PCR Resources

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Polymerase Chain Reaction

Techniques used for replication of a single strand of DNA to provide sufficient quantity for other applications.

Subcategories

• Amplified Fragment Length Polymorphism
• In Situ PCR
• RNA to DNA Amplification
• Real Time PCR
• Software

Resources in This Category

• Attotron Biosensor Corporation

  http://www.attotron.com/
  Research and development company for development of biosensors and related products for the research and educational markets.

• BioRad, Amplification, PCR

  http://www.bio-rad.com/B2B/BioRad/product/br_category.jsp?BV_SessionID=@@@@0937418475.1167690030@@@@&BV_EngineID=ccciaddjkighejmcfngcfkmdhkkdfll.0&divName=Life+Science+Research&loggedIn=false&serviceLevel=Lit+Request&lang=English&csel=HQ&catLevel=3&catOID=-32427&isPA=false&categoryPath=/Catalogs/Life+Science+Research/Amplification+%7C+PCR
  Division of BioRad Laboratories that manufactures and sells instruments for PCR, in Hercules, California, USA.

• GeneOhm Sciences

  http://www.geneohm.com/
  Tests on group B Streptococcus and methicillin resistant Staphylococcus aureus by PCR / DNA sequencing.

• GenHunter

  http://www.genhunter.com/
  Manufacturer of material for differential display PCR in Nashville, Tenn USA.

• HiFi DNA

  http://www.hifidna.com/
  HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails.

• Ingenetix GmbH

  http://www.ingenetix.com/
  Develops technology and products for DNA and mRNA research. Also provide DNA testing for the determination of parentage/paternity and custom DNA sequencing, oligonucleotide synthesis, genotyping services, pharmacogenetics and quantitative PCR, in Vienna, Austria.

• Inverse PCR and Cycle Sequencing of P Element Insertions for STS Generation

  http://www.fruitfly.org/about/methods/inverse.pcr.html
  Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA.

• Inverse PCR for Use with Snyder mTn-lacZ/LEU2-based Mutagenesis

  http://labs.fhcrc.org/gottschling/General%20Protocols/ipcr.html
  Protocol by M McMurray, Fred Hutchinson Cancer Research Center, Seattle, Wa. USA.

• Inverse PCR Protocol

  http://www.mcdb.lsa.umich.edu/labs/maddock/protocols/PCR/inverse_pcr_protocol.html
  Step by step protocol, from the web site of the Department of Biology, University of Michigan, USA.

• Kary B. Mullis - Autobiography

  http://nobelprize.org/chemistry/laureates/1993/mullis-autobio.html
  The originator of PCR, from the Nobel e-museum web site.

• Nematode ITS1 Size Variation

  http://nematode.unl.edu/its_id/EXAMPLES/index.htm
  Examples of Restriction Fragment Length Polymorphism (RFLP)electrophoresis slabs for different nematodes, from University of Nebrasca.

• PCR Amplification of cDNA Segments by 2 Stage Nested PCR

  http://www.ncbi.nlm.nih.gov/SNP/snp_viewTable.cgi?type=method&method_id=555
  Protocol from the method database of NIH, USA.

• PCR Animated

  http://users.ugent.be/~avierstr/principles/pcrani.html
  Animation illustrating the principle of PCR, from the University of Ghent, Belgium.

• PCR Gateway

  http://www.horizonpress.com/pcr/
  A directory of PCR techniques, PCR protocols, PCR troubleshooting, PCR websites and online resources from the publisher Horizon Press.

• PCR Guru

  http://www.pcrguru.com/
  A downloadable textbook on PCR setup and optimization, not free.

• PCR Protocol

  http://www.mcdb.lsa.umich.edu/labs/maddock/protocols/PCR/general_pcr_protocol.html
  Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.

• PCR Protocols

  http://www.cas.psu.edu/docs/CASDEPT/VET/jackvh/jvhpcr.html
  Protocols and technical hints, particularly for reverse transcription PCR, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Toxicology Program, Penn State University

• PCR-ELISA and Related

  http://www.btc-bti.com/pcrelisa.htm
  P Zhang, CJ Gebhart, D Burden, GE Duhamel: A low technology alternative to real time PCR, technical article on the site of BT&C, Inc Bridgewater, NJ, USA.

• PrimerDigital

  http://primerdigital.com/
  International biotechnology company specialized in a design service for PCR primers and probes, PCR-based technology development, projects for development of polymorphism and software development.

• Primerfox

  http://www.primerfox.com/
  Free online tool for generation of PCR primers.

• Principle of PCR

  http://users.ugent.be/~avierstr/principles/pcr.html
  Applications in work on aging of Caenorhabditis elegans and phylogeny of nematodes, by Andy Vierstraete, Department of Biology, University of Ghent, Belgium.

• Protocols Online: PCR Protocols

  http://www.protocol-online.org/prot/Molecular_Biology/PCR/
  Extensive collection of PCR protocols and methods from Protocol On Line.

• Rational Primer Design Greatly Improves Differential Display-PCR (DD-PCR)

  http://nar.oxfordjournals.org/content/25/11/2239.full
  Article: D Graf, AG Fisher, M Merkenschlager: Nucl. Acids Res. 25:11 2239-2240.

• Reference in PCR

  http://www.gene-quantification.org/
  Technical aspects of quantitative real-time PCR and RT-PCR. Instruments, kits, dyes, chemistries, and services presented by their manufacturers.

• Roe Laboratory Protocols

  http://www.genome.ou.edu/proto.html
  Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK.

• Single Tube Confirmation PCR Protocol

  http://www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html
  For characterization colonies of transformed clones of Saccharaomyces, from the web site of the Stanford Genome Technology Center, Palo Alto, CA, USA.

• T-DNA Generated Enhancer Traps in Arabidopsis

  http://www.dartmouth.edu/~tjack/
  Application of inverse PCR, partial genomic libraries and TAIL-PCR in cloning flanking, at the Department of Biological Sciences, Dartmouth College, Hanover, NH.

• The PCR Encyclopedia

  http://www.pcr-encyclopedia.com/
  Describes plans for a free encyclopedia dedicated to the polymerase chain reaction (PCR).

• The Web Guide of PCR

  http://www.pcrlinks.com/
  List of links and forum on the subject and related methodology. Set up and maintained by SJ Krivokapich, National University of Misiones, Argentina.

• Thermostable DNA Polymerases

  http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/enzymes/hotpolys.html
  Discussion of their origin and briefly their properties. From the web site of Colorado State University.

• Which DNA Marker for Which Purpose?

  http://webdoc.sub.gwdg.de/ebook/y/1999/whichmarker
  Compendia of the Research Project "Development, optimisation and validation of molecular tools for assessment of biodiversity in forest trees", European Union DGXII Biotechnology FW IV Research Programme. From the web site of the University Library, Göttingen.

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